We provide stem cell lines for use in research, drug discovery and development and pre-clinical studies. Our stem cell lines include adult cells known as MSCs (mesenchymal stem cells) that are gaining considerable support for clinical efficacy and safety in the treatment of a large variety of conditions including injuries to bone and soft tissue and treatment of numerous other conditions resulting from cellular degeneration.
Our MSCs & Derivatives include human cord blood-derived MSCs as native cells without any added label and also a variety of derivatives of these cells including various fluorescent formats, and an Oct3/4 transfected cell line that shows increased potency compared to native MSCs. Our fluorescent MSCs include membrane-labeled fluorescein and rhodamine cells, GFP-expressing and green-fluorescent nanoparticle labeled MSCs. We are currently developing SPIO (supra paramagnetic iron oxide), nanoparticle-labeled human MSCs and plan to introduce dual labeled MSCs. SPIO-labeled cells enable MRI and NMR imaging procedures
We have recently added a series of terminally differentiated cells derived from MSCs. These are produced under controlled conditions resulting in reproducible cellular characteristics reflecting native tissues. Our present offering includes chondrocytes and osteoblasts, while endothelial cells are presently in development. These products are suitable for in-vitro cell-based assays for drug discovery and development and for regenerative medicine studies as well. Our terminally differentiated cells are available in a variety of formats as well, including native, fluorescein/rhodamine-labeled and SPIO-labeled as well. We are capable of providing custom cell lines as well, for example, luciferase-reporter genes for specific applications.
We also provide a well-characterized human primary pancreatic cell line for various research applications including generation of iPSCs, study of CAFs (cancer-associated fibroblasts) and vaccine production.
All of our cell lines are supported by media manufactured by Vitro Biopharma as well (See link to Cell Culture Media)
Our cell lines are well-characterized with regard to species authentication using sensitive PCR methods to quantify non-conserved genes including COX1, Cytochrome B and actin. We also utilize karyotyping to authenticate our cell lines. Adventitious agents are also tested negative by sensitive PCR methods including known viral contaminants and mycoplasma. Performance is assured by rigorous testing of viability, growth rate and differentiation capacity for formation of chondrocytes, adipocytes and osteoblasts. Our MSCs and derivative are characterized with regard to phenotypic cluster designation antigens.